Coding

Part:BBa_K1137000:Design

Designed by: Matthew Deyell   Group: iGEM13_Paris_Bettencourt   (2013-09-23)

M. Smegmatis SirA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 152
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 711
    Illegal AgeI site found at 994
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

High degree of homology to SirA from M. Tuberculosis


Source

Synthesized from M. Smegmatis genome sequence and Codon Optimized for expression in E. coli with JCat

References

Pinto et al. 2007. Sulfite Reduction in Mycobacteria doi: 10.1128/JB.00487-07